Quick Overview
100 rxns
100 mL
100 mL
Details
The gPURE DNA Isolation Kit offers a simple and gentle reagent DNA precipitation method for isolating high molecular weight genomic, mitochondrial, or viral DNA suitable for archiving or sensitive downstream applications. This highly versatile solution based system can be scaled proportionately in order to satisfy larger sample volumes providing a convenient sample-storage procedure with minimal hands on time. Initially cells are lysed in the presence of detergents and a proprietary DNA stabilization solution followed by RNase A treatment. Once proteins and other contaminants are removed DNA is precipitated then rehydrated. The high quality extracted DNA is ready for use in a variety of downstream applications.
gPURE DNA Isolation Kits are tested on a lot-to-lot basis by isolating DNA from (3-5 x 106) cultured cells. The isolated DNA (5-15 µg with an A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and analyzed by electrophoresis.
Features:
•Sample Size: scalable for cultured cells
•Expectant Yield: high quality (A260/A280=1.8-2.0)
gPURE DNA Isolation Kits are tested on a lot-to-lot basis by isolating DNA from (3-5 x 106) cultured cells. The isolated DNA (5-15 µg with an A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and analyzed by electrophoresis.
Features:
•Sample Size: scalable for cultured cells
•Expectant Yield: high quality (A260/A280=1.8-2.0)
Additional Information
| UNSPSC | 41105504 |
|---|---|
| Applications | Genomic DNA Extraction |
| Nucleic Acid Binding Capacity | N/A |
| Nucleic Acid | DNA |
| Number of Preps | N/A |
| Protocol Time | N/A |
| Sample Type | Cultured Cells |
| Target Length | N/A |
| Yield | 5-15 µg isolated DNA |
