Mfg Part Number
QBD-10199-1000
Vector Labs QBD-10199-1000 dPEG®₄-biotin acid - 1000 mg (1/EA)
Quick Overview
NHS-dPEG₄-biotin
$725.88
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dPEG®4 biotin acid, product number QBD-10199, is a biotinylation reagent containing a single molecular weight tetraethylene glycol (PEG) spacer containing biotin on one end and a propionic acid moiety on the other. The terminal propionic acid group couples to amines directly using a carbodiimide such as EDC, and it can be functionalized as an N-hydroxysuccinimidyl (NHS) or 2,3,5,6-tetrafluorophenyl (TFP) ester for reaction with amines.
Amphiphilic dPEG®4 biotin acid can be dissolved both in water or aqueous buffer and in organic solvents. When used as a label, it does not cause aggregation or precipitation of biomolecules even when excess label is used. Using 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) chemistry, QBD-10199 can be coupled directly to free primary amines in aqueous media. These free amines can be on a protein, peptide, or the treated surface of a nanoparticle. The linker length of dPEG®4 biotin acid is slightly longer than hydrophobic LC-biotin and demonstrates superior performance over LC-biotin due to its flexiblity and water solubility.
Product number QBD-10199 is the precursor product of our popular products QBD-10198, NHS-dPEG®4-biotin, and QBD-10008, Biotin-dPEG®4-TFP ester. Many applications use this product to take advantage of the strong avidin-biotin binding interaction. Such applications include single-cell imaging of protein secretion, developing biosensors, isolating specific cell types by separation with magnetic beads, characterizing and understanding the streptavidin-biotin interaction, creating affinity-based probes, and assembling supramolecular nanostructures.
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161700 |
| Reagent Application | Bioconjugation |
| Reagent Size | 1000 mg |