Mfg Part Number
QBD-10631-1000
Vector Labs QBD-10631-1000 Bis-MAL-Lysine-dPEG®₄-TFP ester - 1000 mg (1/EA)
Quick Overview
Lipoamido-dPEG₄-TFP ester - 100 mg
$1,510.43
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Bis-MAL-Lysine-dPEG®4-TFP ester, product number QBD-10631, is a unique crosslinker from Vector Laboratories, Inc. The alpha (α) and epsilon (ε) amines of lysine are functionalized with sulfhydryl-reactive maleimidopropyl groups. The carboxylic acid tail of lysine is conjugated to a single molecular weight, discrete polyethylene glycol (dPEG®) spacer containing an amine-reactive 2,3,5,6-tetrafluorophenyl (TFP) ester. The distance from the carbonyl carbon of the dPEG® spacer to the reactive site of the α-amine-conjugated maleimide group is 25 atoms (18.773 Å) long. The distance from the carbonyl carbon of the dPEG® spacer to the reactive site of the ε-amine-conjugated maleimide group is 29 atoms (26.105 Å) long.
TFP esters react with primary and secondary amines to form stable amide bonds like the more widely used N-hydroxysuccinimidyl (NHS) esters. However, TFP esters demonstrate superior hydrolytic stability and higher reactivity with amines than NHS esters. The optimum pH for this reaction is 7.5 – 8.0. Thus, the product functions as a heterobifunctional crosslinker.
By design, the two maleimide groups of Bis-MAL-Lysine-dPEG®4-TFP ester can be used to bridge disulfides. For example, this capability may be useful in antibodies following reduction of the disulfide groups. Moreover, the two maleimide groups can be used to dimerize small molecules or peptides, each of which possesses a single free thiol group. At pH 6.5 – 7.5, maleimide groups react chemoselectively with sulfhydryls. However, above pH 7.5, chemoselectivity is lost, as maleimide groups also will react with free amines.
Possible uses for Bis-MAL-Lysine-dPEG®4-TFP ester include the construction of antibody-drug conjugates (ADCs); immobilization of antibodies or antibody fragments on solid supports; and dimerization of sulfhydryl-containing peptides followed by crosslinking to an amine-modified surface.
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161700 |
| Reagent Application | Bioconjugation |
| Reagent Size | 1000 mg |