Mfg Part Number
QBD-10642-1000
Vector Labs QBD-10642-1000 Lipoamido-dPEG®₈-TFP ester - 1000 mg (1/EA)
Quick Overview
Lipoamido-dPEG₂₄-TFP ester - 100 mg
$1,389.99
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Lipoamido-dPEG®8-TFP ester, product number QBD-10642, is a single molecular weight, discrete PEG (dPEG®) modifier of gold and silver surfaces, for example, on nanoparticles. The lipoic acid moiety on one end of the 36-atoms-long dPEG® spacer forms dative bonds with metals like gold and silver. The TFP ester reacts with free amines to form free amines, optimally in the pH range 7.5 – 8.0. The distance from the disulfide bond in the lipoic acid ring to the carbonyl carbon adjacent to the 2,3,5,6-tetrafluorophenol (TFP) ester, the distance is 36 atoms (42.7 Å). Applications for this product include creating self-assembled monolayers (SAMs) on metal surfaces and crosslinking and immobilizing peptides and proteins to the surfaces of metal nanoparticles.
Lipoic acid, also known as alpha-lipoic acid, α-lipoic acid, and thioctic acid, consists of a five-membered ring containing a disulfide attached to a pentanoic acid group. As a naturally occurring antioxidant, lipoic acid functions as a cofactor in several enzyme systems. It is a bidentate thiol ligand that forms two stable dative bonds per lipoic acid group with gold, silver, and other metals. These dative bonds form with oxidized lipoic acid or reduced dihydrolipoic acid (DHLA). Because it forms two dative bonds on metal surfaces, lipoic acid is more stable than PEGylation reagents containing a single thiol. This increased stability prevents lipoic-acid-functionalized dPEG® products from being oxidized off the metal surface. The lipoic acid group forms dative bonds with metals with or without reduction to DHLA. If reduction to DHLA is preferred, Tris(2-carboxyethyl)phosphine (TCEP) will rapidly reduce lipoic acid to DHLA.
TFP esters react more readily with amines and possess better hydrolytic stability than N-hydroxysuccinimidyl (NHS) esters. The superiority of PEG-TFP esters over NHS esters is documented in the scientific literature. When using this product, it is typical to conjugate the carboxyl end of the dPEG® to an amine to form an amide bond before forming a dative bond between a metal surface and the lipoic acid moiety of Lipoamido-dPEG®8-TFP ester. The TFP ester reacts optimally at pH 7.5 – 8.0, but it will also react with amines at pH values above and below this range.
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161700 |
| Reagent Application | Bioconjugation |
| Reagent Size | 1000 mg |