Mfg Part Number
QBD-10776-100
Vector Labs QBD-10776-100 dPEG®₄₈-biotin acid - 100 mg (1/EA)
Quick Overview
dPEG₄₈-biotin acid
$363.44
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dPEG®48 biotin acid, product number QBD-10776, is a biotinylation reagent containing a long (157 atoms), single molecular weight, discrete polyethylene glycol (dPEG®) spacer that is functionalized with biotin on one end and a propionic acid moiety on the other. The terminal propionic acid group couples to amines directly using a carbodiimide such as EDC, and it can be functionalized as an N-hydroxysuccinimidyl (NHS) or 2,3,5,6-tetrafluorophenyl (TFP) ester for reaction with amines.
Amphiphilic dPEG®48 biotin acid is soluble in water, aqueous buffers, and organic solvents. When used as a label, it does not cause aggregation or precipitation of biomolecules even when excess label is used. Using 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) chemistry, QBD-10776 can be coupled directly to free primary amines in aqueous media. These free amines can be on a protein, peptide, or the treated surface of a nanoparticle. The linker length of dPEG®48 biotin acid is longer than the linker length of LC-biotin and longer than the linker length of LC-LC-biotin. The performance characteristics of dPEG®48-biotin acid are quite superior to both LC-biotin and LC-LC-biotin.
Many applications use this product to take advantage of the strong avidin-biotin binding interaction. Such applications include single-cell imaging of protein secretion, developing biosensors, isolating specific cell types by separation with magnetic beads, characterizing and understanding the streptavidin-biotin interaction, creating affinity-based probes, and assembling supramolecular nanostructures.
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161700 |
| Reagent Application | Bioconjugation |
| Reagent Size | 100 mg |