Mfg Part Number
QBD-11812-1
Vector Labs QBD-11812-1 DBCO-dPEG®₁₂-carboxyfluorescein - 1 mg (1/EA)
Quick Overview
DBCO-dPEG₁₂-carboxyfluorescein - 5 mg
$237.47
Order will ship within 1-3 business days
DBCO-dPEG®12-carboxyfluorescein, product number QBD-11812, permits fluorescein labeling of peptides, proteins, and other molecules using strain-promoted azide-alkyne cycloaddition (SPAAC). This compound combines the xanthene dye, 5(6)-carboxyfluorescein, with the popular click chemistry alkyne dibenzylcyclooctyne (DBCO) through a medium-length (40 atoms, 47.6 Å), single molecular weight, discrete PEG (dPEG®) spacer. The carboxyfluorescein label has an excitation wavelength (λex) of 498 nanometers (nm), and an emission wavelength (λem) of 528 nm. The molar absorptivity (ε) is 7.96×104 M-1 cm-1 at pH 8.0 and 498 nm.
Fluorescein and its derivatives are among the most popular dyes in use today for labeling proteins and cells. Unfortunately, these dyes are hydrophobic, and care must be taken to avoid over-labeling because over-labeling can result in quenching and possibly aggregation and precipitation of the conjugates through non-specific hydrophobic interactions.
DBCO is an increasingly popular choice for SPAAC chemistry, also known as “”copper-free click chemistry”” because it avoids the use of cytotoxic copper(I) ions and allows reactions to occur in and with living cells. Although DBCO may react with free sulfhydryl groups under some conditions, it is highly selective for azides. Because azides must be introduced artificially into proteins, DBCO is considered a bioorthogonal click chemistry reagent.
The introduction of a flexible dPEG® spacer between carboxyfluorescein and DBCO increases the molecule’s hydrodynamic volume and imparts water solubility to DBCO-dPEG®12-carboxyfluorescein. Thus, DBCO-dPEG®12-carboxyfluorescein provides a highly selective, flexible, water-soluble way to label proteins, peptides, and cells with carboxyfluorescein using a bio-orthogonal reagent designed to simplify analysis and characterization of the conjugate.
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161700 |
| Reagent Application | Bioconjugation |
| Reagent Size | 1 mg |