Mfg Part Number
Ab00101-6.1
Vector Labs Ab00101-6.1 Anti-His tag (C-term) [3D5], Rat IgG1, kappa (200 μg)
Quick Overview
Purified his tagged Fab fragment.
$517.40
Order will ship within 1-3 business days
| Product Class: | Purified |
|---|---|
| Expected Species Reactivity: | Species independant |
| Clone ID: | 3D5 |
| Antibody Species: | Rat |
| Isotype: | IgG1 |
| LC Format: | kappa |
| Synonyms: | polyhistidine tag |
| Amount: | 200 μg |
| Application Codes Clone: | WB; ELISA; FACS; FC |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2b |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This is a chimeric rat antibody, based on the original mouse IgG2b format, created for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody binds to carboxy-terminal oligo-histidine tags (6x His tags) of fusion proteins. It also has affinity for internal His-tags but no affinity for N-terminal His-tags. |
| Application Notes: | This antibody was successfully used for ELISA, WB, and FACS. Monomeric and dimeric scFv versions of the antibody were generated and described (Lindner et al., 1997; PMID: 8994661). The scFv version of this antibody was engineered into a lentiviral vector system to confer specificity for cells displaying the His tag on their surface, enabling targeted gene transfer into HT1080-anti-His cells (Friedel et al., 2015; PMID: 25715658). This antibody was used in FC to detect Canine IL-17A in Canine IL-17A-transfected HEK293 A cells (Akiyama et al., 2019; PMID: 30885301). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |