Mfg Part Number
Ab00102-1.6
Vector Labs Ab00102-1.6 Anti-Fluorescein [4-4-20 (enhanced)], Mouse Fab fragment, His-Tagged, kappa (200 μg)
Quick Overview
The original antibody was generated by immunizing BALB/c mice with fluorescein I-keyhole limpet hemocyanin (FlI-KLH).
$517.40
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| Product Class: | Purified |
|---|---|
| Expected Species Reactivity: | Species independent |
| Clone ID: | 4-4-20 (enhanced) |
| Antibody Species: | Mouse |
| Isotype: | Fab fragment |
| LC Format: | kappa |
| Heavy Chain Modification: | His-Tagged |
| Synonyms: | FL; FlI; FITC; Fluorescein isothiocyanate; fluorescein isothiocyanate, isomer 1; Fluorescyl; Resorcinolphthalein; C.I. 45350; solvent yellow 94; D & C yellow no. 7; Angiofluor; Japan yellow 201; Soap yellow; 3′,6′-dihydroxyspiro[isobenzofuran-1(3H),9′-[9H]xanthen]-3-one |
| Amount: | 200 μg |
| Application Codes Clone: | negative control; in vivo |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 10556036 |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | scFv |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This is a recombinant chimeric Fab fragment created as part of a panel offering antibodies of the same specificity in different formats (species, isotype, subtype and modified versions) for use as isotype controls. |
| Specificity Statement: | This antibody is specific for fluorescein I. |
| Application Notes: | This antibody, a humanized, enhanced scFv of the original mouse IgG2a antibody, was constructed by grafting the CDRs of the original antibody onto the humanized 4D5 anti-HER2 antibody framework, and subsequently enhancing it with respect to folding and stability via randomization in the form of DNA shuffling, as well as oligonucleotides encoding mutations at defined positions (Jung et al., 1999; PMID: 10556036). The original antibody was tested against FlI-BSA. The enhanced antibody fragment was tested against FITC-BSA. This antibody can be used as an isotype control in both in vitro and in vivo applications. This antibody is not conjugated to fluorescein. |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |