Vector Labs Ab00110-1.1 Anti-CD134 [OX86], Mouse IgG1, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Expected Species Reactivity: Mouse
Clone ID: OX86
Isotype: IgG1
Synonyms: OX40; OX-40; Tumor necrosis factor receptor superfamily member 4; OX40 antigen; OX40L receptor; CD134; Tnfrsf4; OX-86
Amount: 200 μg
Application Codes Clone: FC; agonist; IF; ELISA
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This is a chimeric antibody created for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody recognises murine OX40 (CD134), a cell surface antigen expressed only by activated T lymphocytes. It is a member of TNFR-superfamily of receptors and acts as a costimulatory molecule involved in long-term T-cell immunity.
Application Notes: OX86 antibody, in its original rat IgG1 format, acts as an OX40 agonist in an Fc-dependent manner and has been widely used in studies investigating the role of OX40 in tumor inhibition (Hirschhorn-Cymerman et al., 2009). Studies comparing two recombinant murine Fc versions (IgG1 and IgG2a) elucidated the importance of the FcR engagement in the antitumor activity of this antibody; the study demonstrated that a stronger murine Fc (in an IgG2a version) was more effective in inducing a CT26 syngeneic tumor regression as well as it led to a stronger Treg depletion in the tumor in comparison with a murine IgG1 version (Metzger et al., 2016). In another study two versions of the OX86 antibody (with a fully functional Fc fragment and with an impaired Fc) and showed that Fc-dependent activity was necessary in inducing systemic antitumor activity (Sagiv-Barfi et al., 2018). FC: This antibody was also used in flow cytometry to stain mouse CD4 and CD8 single-positive cells (al-Shamkhani et al., 1996). FC: Blanquiceth et al. (2016) used this antibody in flow cytometry as well to analyse cells coming from mediastinal lymph nodes and lung tissue. IHC: OX86 antibody was utilised in immunohistochemistry staining of gut tissue (Peyer's patches and lamina propria samples) in order to identify OX40-positive cells in colitic mice (Higgins et al., 1999).

Product Class:	Purified
Expected Species Reactivity:	Mouse
Clone ID:	OX86
Isotype:	IgG1
Synonyms:	OX40; OX-40; Tumor necrosis factor receptor superfamily member 4; OX40 antigen; OX40L receptor; CD134; Tnfrsf4; OX-86
Amount:	200 μg
Application Codes Clone:	FC; agonist; IF; ELISA
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This is a chimeric antibody created for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody recognises murine OX40 (CD134), a cell surface antigen expressed only by activated T lymphocytes. It is a member of TNFR-superfamily of receptors and acts as a costimulatory molecule involved in long-term T-cell immunity.
Application Notes:	OX86 antibody, in its original rat IgG1 format, acts as an OX40 agonist in an Fc-dependent manner and has been widely used in studies investigating the role of OX40 in tumor inhibition (Hirschhorn-Cymerman et al., 2009). Studies comparing two recombinant murine Fc versions (IgG1 and IgG2a) elucidated the importance of the FcR engagement in the antitumor activity of this antibody; the study demonstrated that a stronger murine Fc (in an IgG2a version) was more effective in inducing a CT26 syngeneic tumor regression as well as it led to a stronger Treg depletion in the tumor in comparison with a murine IgG1 version (Metzger et al., 2016). In another study two versions of the OX86 antibody (with a fully functional Fc fragment and with an impaired Fc) and showed that Fc-dependent activity was necessary in inducing systemic antitumor activity (Sagiv-Barfi et al., 2018). FC: This antibody was also used in flow cytometry to stain mouse CD4 and CD8 single-positive cells (al-Shamkhani et al., 1996). FC: Blanquiceth et al. (2016) used this antibody in flow cytometry as well to analyse cells coming from mediastinal lymph nodes and lung tissue. IHC: OX86 antibody was utilised in immunohistochemistry staining of gut tissue (Peyer's patches and lamina propria samples) in order to identify OX40-positive cells in colitic mice (Higgins et al., 1999).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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