Mfg Part Number
Ab00294-1.4
Vector Labs Ab00294-1.4 Anti-Phosphotyrosine [PY20], Mouse IgG1, Fc Silent™, kappa (200 μg)
Quick Overview
Phosphotyrosine conjugated to Keyhole limpet hemocyanin (KLH).
$517.40
Order will ship within 1-3 business days
| Product Class: | Purified |
|---|---|
| Expected Species Reactivity: | Species independent |
| Clone ID: | PY20 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | p-Tyr; Phospho-tyrosine; p-Y |
| Amount: | 200 μg |
| Application Codes Clone: | WB; IF; IHC-P; IHC-FoFr; IP |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | PMID: 1706720 |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2b |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This is a chimeric antibody created for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | PY20 recognizes phospho-tyrosine and phosphotyrosine containing proteins with a reported Kd of 500 nM for the original clone.Whilst phospho-Phenylalanine (Kd = 4 uM M), phospho-Serine (>100 mM) & phospho-Threonine (100 mM) are also detected, the differences in affinity make this antibody primarily phospho-tyrosine specific. |
| Application Notes: | Protein phosphorylation is a key mechanism of signal transduction in Eukaryotes and Prokaryotes. PY20 has been a crucial antibody in the investigation of these processes. An excellent comparison of anti-phosphotyrosine antibodies can be found here . |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |