Mfg Part Number
Ab00295-2.0-VXX
Vector Labs Ab00295-2.0-VXX Anti-Ly6G [1A8], Mouse IgG2a, kappa (25 mg)
Quick Overview
LEW/N rats immunized with 1.5x10E8 Ly-6G-transfected EL4J cells
$3,286.40
Order will ship within 1-3 business days
| Product Class: | VivopureX |
|---|---|
| Expected Species Reactivity: | Mouse |
| Clone ID: | 1A8 |
| Synonyms: | LGR-1 ; y-6G; GR1; Lymphocyte antigen 6G |
| Amount: | 25 mg |
| Application Codes Clone: | FC; depletion |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS only. |
| Original Format: | IgG2a |
| Storage Temperature: | All VivopureX products are formulated in PBS only without addition of preservatives. To ensure optimal storage and prevent microbial contamination, only open and dispense under sterile conditions. |
| Chimeric Use Statement: | This is a murinised antibody to reduce immunogenicity in vivo, however with an isotype chosen to promote depletion of targeted cells via ADCC and CDC |
| Specificity Statement: | Recognises the murine GPI-anchored protein Ly6G, a marker of granulocyte differentiation, maturation and, together with Ly6C, a component of the Granulocyte Receptor-1 Antigen (Gr-1). Unlike another commonly used clone, RB6-8C5, 1A8 does not bind Ly6C. |
| Application Notes: | Neutrophils are crucial components of our immune system. Ly6G is highly expressed on neutrophils, which allows the 1A8 clone to be used not only in detection of neutrophils, but also in selective depletion in vivo. It may also be used as a negative selection marker for bone-marrow derived macrophages, which should be Ly6G(-) and F4/80 (+) (see our F4/80 range of antibodies) . The mouse IgG2a version of the antibody, recombinantly produced by Absolute Antibody, could deplete neutrophils in mice more efficiently than the rat version of the antibody. Further, this version increased depleting effector function, and prevented the production of anti-rat antibodies (Olofsen et al., 2022; PMID: 36359801). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |