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Mfg Part Number

Ab00295-2.3-VPT

Vector Labs Ab00295-2.3-VPT Anti-Ly6G [1A8], Mouse IgG2a, Fc Silent™, kappa (10 mg)

VECTOR LABS

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Quick Overview

LEW/N rats immunized with 1.5x10E8 Ly-6G-transfected EL4J cells

$1,506.70

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Description

Product Class: Vivopure
Expected Species Reactivity: Mouse
Clone ID: 1A8
Heavy Chain Modification: Fc Silent™
Synonyms: LGR-1 ; y-6G; GR1; Lymphocyte antigen 6G
Amount: 10 mg
Application Codes Clone: FC; depletion
Shipping Temperature: Wet Ice
Buffer Composition: PBS only, with >98% antibody purity and <1 EU/mg guaranteed.
Original Format: IgG2a
Storage Temperature: All vivopure products are formulated in PBS only without addition of preservatives. To ensure optimal storage and prevent microbial contamination, only open and dispense under sterile conditions.
Chimeric Use Statement: This is a murinised antibody to reduce immunogenicity in vivo and was created using our proprietary Fc Silent™ engineered Fc domain, containing key point mutations that abrogate binding of Fc Receptor and complement.
Specificity Statement: Recognises the murine GPI-anchored protein Ly6G, a marker of granulocyte differentiation, maturation and, together with Ly6C, a component of the Granulocyte Receptor-1 Antigen (Gr-1). Unlike another commonly used clone, RB6-8C5, 1A8 does not bind Ly6C.
Application Notes: Neutrophils are crucial components of our immune system. Ly6G is highly expressed on neutrophils, which allows the 1A8 clone to be used not only in detection of neutrophils, but also in selective depletion in vivo. It may also be used as a negative selection marker for bone-marrow derived macrophages, which should be Ly6G(-) and F4/80 (+) (see our F4/80 range of antibodies) . The mouse IgG2a version of the antibody, recombinantly produced by Absolute Antibody, could deplete neutrophils in mice more efficiently than the rat version of the antibody. Further, this version increased depleting effector function, and prevented the production of anti-rat antibodies (Olofsen et al., 2022; PMID: 36359801).

Product Class:	Vivopure
Expected Species Reactivity:	Mouse
Clone ID:	1A8
Heavy Chain Modification:	Fc Silent™
Synonyms:	LGR-1 ; y-6G; GR1; Lymphocyte antigen 6G
Amount:	10 mg
Application Codes Clone:	FC; depletion
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS only, with >98% antibody purity and <1 EU/mg guaranteed.
Original Format:	IgG2a
Storage Temperature:	All vivopure products are formulated in PBS only without addition of preservatives. To ensure optimal storage and prevent microbial contamination, only open and dispense under sterile conditions.
Chimeric Use Statement:	This is a murinised antibody to reduce immunogenicity in vivo and was created using our proprietary Fc Silent™ engineered Fc domain, containing key point mutations that abrogate binding of Fc Receptor and complement.
Specificity Statement:	Recognises the murine GPI-anchored protein Ly6G, a marker of granulocyte differentiation, maturation and, together with Ly6C, a component of the Granulocyte Receptor-1 Antigen (Gr-1). Unlike another commonly used clone, RB6-8C5, 1A8 does not bind Ly6C.
Application Notes:	Neutrophils are crucial components of our immune system. Ly6G is highly expressed on neutrophils, which allows the 1A8 clone to be used not only in detection of neutrophils, but also in selective depletion in vivo. It may also be used as a negative selection marker for bone-marrow derived macrophages, which should be Ly6G(-) and F4/80 (+) (see our F4/80 range of antibodies) . The mouse IgG2a version of the antibody, recombinantly produced by Absolute Antibody, could deplete neutrophils in mice more efficiently than the rat version of the antibody. Further, this version increased depleting effector function, and prevented the production of anti-rat antibodies (Olofsen et al., 2022; PMID: 36359801).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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