Mfg Part Number
Ab00295-23.0
Vector Labs Ab00295-23.0 Anti-Ly6G [1A8], Rabbit IgG, kappa (200 μg)
Quick Overview
LEW/N rats immunized with 1.5x10E8 Ly-6G-transfected EL4J cells
$517.40
Order will ship within 1-3 business days
| Product Class: | Purified |
|---|---|
| Expected Species Reactivity: | Mouse |
| Clone ID: | 1A8 |
| Synonyms: | LGR-1 ; y-6G; GR1; Lymphocyte antigen 6G |
| Amount: | 200 μg |
| Application Codes Clone: | FC; depletion |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2a |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | Recognises the murine GPI-anchored protein Ly6G, a marker of granulocyte differentiation, maturation and, together with Ly6C, a component of the Granulocyte Receptor-1 Antigen (Gr-1). Unlike another commonly used clone, RB6-8C5, 1A8 does not bind Ly6C. |
| Application Notes: | Neutrophils are crucial components of our immune system. Ly6G is highly expressed on neutrophils, which allows the 1A8 clone to be used not only in detection of neutrophils, but also in selective depletion in vivo. It may also be used as a negative selection marker for bone-marrow derived macrophages, which should be Ly6G(-) and F4/80 (+) (see our F4/80 range of antibodies) . The mouse IgG2a version of the antibody, recombinantly produced by Absolute Antibody, could deplete neutrophils in mice more efficiently than the rat version of the antibody. Further, this version increased depleting effector function, and prevented the production of anti-rat antibodies (Olofsen et al., 2022; PMID: 36359801). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |