Mfg Part Number
Ab00337-6.4
Vector Labs Ab00337-6.4 Anti-Rhodopsin [Rho 1D4], Rat IgG1, Fc Silent™, kappa (200 μg)
Quick Overview
Bleached bovine rod outer segment (ROS).
$517.40
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| Product Class: | Purified |
|---|---|
| Expected Species Reactivity: | Mouse; Rat; Cow; Human, Zebrafish, Amphibians |
| Clone ID: | Rho 1D4 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | Rho-tag; Visual Purple |
| Amount: | 200 μg |
| Application Codes Clone: | Radioimmune labeling; WB; ELISA; IP; IF; IHC |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rat antibody was made using the variable domain sequences of the original mouse IgG1 format for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | The antibody binds to the C-terminus of rhodopsin on the cytoplasmic side of disk membranes. The epitope has the following amino acid sequence: 'TETSQVAPA'. A limitation of the 1D4 tag, however, is that it has to be placed at the C-terminus of a protein. This is because the Rho1D4 monoclonal antibody requires a free carboxylate group for high affinity binding. Amidation of the carboxyl group lowers its immunoreactivity to the Rho1D4 antibody by over 100 fold. |
| Application Notes: | The antibody binds to the C-terminus of rhodopsin on the cytoplasmic side of disk membranes. Rhodopsin is the major pigment in rod photoreceptors and belongs to the family of G-protein-coupled receptors (GPCRs). The linear epitope (TETSQVAPA) can be used as a protein tag. |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |