Mfg Part Number
Ab00404-23.3
Vector Labs Ab00404-23.3 Anti-Beta-Tubulin [S11B], Rabbit IgG, Fc Silent™, lambda (200 μg)
Quick Overview
The original antibody was generated via phage display from the Griffin.1 library by screening against Microtubule-Binding Proteins (MBPs) derived from HeLa S3 cells.
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| Product Class: | Purified |
|---|---|
| Clone ID: | S11B |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | TUBB; β-tubulin |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; WB; IF |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | scFv |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This full-length, chimeric rabbit antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for human beta-tubulin (no cross-reactivity with alpha-tubulin). Tubulin is the major constituent of microtubules. The alpha chain has a non-exchangeable GTP-binding site. Tubulin is the major constituent of microtubules. The beta-chain has an exchangeable GTP-binding site. |
| Application Notes: | This antibody's original format (human scFv) was successfully used in a Western blot, and its anti-myc (9E10) complexed version was successfully used for in vitro immunofluorescence staining of β-tubulin in HeLa cells (Nizak et al., 2003; PMID: 14617357). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |