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Mfg Part Number

Ab00414-10.7

Vector Labs Ab00414-10.7 Anti-Carbonic Anhydrase IX [M75], Human F(ab)2, AbFab2™ His-Tagged, kappa (100 μg)

VECTOR LABS

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Quick Overview

The original antibody was generated by immunizing BALB/C mice with MaTu-infected HeLa cells.

$692.90

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Description

Product Class: Purified
Clone ID: M75
Heavy Chain Modification: AbFab2™ His-Tagged
Synonyms: CA9; CAIX; MN/CA IX; endogenous protein p54/58N
Amount: 100 μg
Application Codes Clone: IB; WB; IF; FC; ELISA; ITC; IHC; Crystallography; Blocking
Shipping Temperature: Wet Ice
Origin Pub PMID: 18041760
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG2b
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is specific for the sequence PGEEDLP, which is repeated several times between the 61st and 96th aa residues of human carbonic anhydrase IX's PG domain.
Application Notes: The original format of this antibody (mouse IgG2b) was characterized by and used in IB (WB), IF, and FC assays (Pastoreková et al., 1992; PMID: 1312272). This antibody's epitope has been determined by a competitive ELISA against the peptide APGEEDLPA. Furthermore, it was used for WB and was shown to blocks the capacity of immobilized MN/CA IX to attach cells (Závada et al., 2000; PMID: 10839295). The Fab version of this antibody was crystallized and analyzed. Additionally, an scFv version of this antibody was created, and its thermodynamic parameters against the peptide DLPGEEDLPGEE were evaluated via ITC. Its K_d was determined to be 0.42 μM (Král et al., 2008; PMID: 18041760). This antibody was used for WB in breast cell lines and for IHC in xenograft tumors grown from PC-3 cells in mice (Li et al., 2009; PMID: 19538935).

Product Class:	Purified
Clone ID:	M75
Heavy Chain Modification:	AbFab2™ His-Tagged
Synonyms:	CA9; CAIX; MN/CA IX; endogenous protein p54/58N
Amount:	100 μg
Application Codes Clone:	IB; WB; IF; FC; ELISA; ITC; IHC; Crystallography; Blocking
Shipping Temperature:	Wet Ice
Origin Pub PMID:	18041760
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG2b
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody is specific for the sequence PGEEDLP, which is repeated several times between the 61st and 96th aa residues of human carbonic anhydrase IX's PG domain.
Application Notes:	The original format of this antibody (mouse IgG2b) was characterized by and used in IB (WB), IF, and FC assays (Pastoreková et al., 1992; PMID: 1312272). This antibody's epitope has been determined by a competitive ELISA against the peptide APGEEDLPA. Furthermore, it was used for WB and was shown to blocks the capacity of immobilized MN/CA IX to attach cells (Závada et al., 2000; PMID: 10839295). The Fab version of this antibody was crystallized and analyzed. Additionally, an scFv version of this antibody was created, and its thermodynamic parameters against the peptide DLPGEEDLPGEE were evaluated via ITC. Its K_d was determined to be 0.42 μM (Král et al., 2008; PMID: 18041760). This antibody was used for WB in breast cell lines and for IHC in xenograft tumors grown from PC-3 cells in mice (Li et al., 2009; PMID: 19538935).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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