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Mfg Part Number

Ab00415-1.4

Vector Labs Ab00415-1.4 Anti-Hairpin DNA [DNA-1], Mouse IgG1, Fc Silent™, kappa (200 μg)

VECTOR LABS

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Quick Overview

The original format of this antibody was isolated from a phage display derived from MRL/lpr mice (autoimmune mice). The library was panned against single-stranded calf thymus DNA.

$517.40

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Description

Product Class: Purified
Clone ID: DNA-1
Heavy Chain Modification: Fc Silent™
Synonyms: dT5; Oligo(dT); Oligo (dT); poly(dT); poly (dT)
Amount: 200 μg
Application Codes Clone: IP; ELISA; Crystallography; ITC
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: Fab
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement: This antibody is specific for dT5, with high specificity to sequences greater than 5 nucleotides. It primarily recognizes the thymine DNA base, but it also binds to oligo(dT)15 nucleotides or greater and oligo(dT) of length three to five nucleotides. The antibody also recognizes the abbreviated hairpin sequence LIG5-14.
Application Notes: The original format of this antibody (Fab) was generated and characterized. It was shown to bind DNA in an IP assay. and its binding affinity was determined to be (4.4±2.4) × 10^-8 M (Calcutt et al., 1993; PMID: 7506692). Fluorescence titration of the antibody with dT5 was performed, and a Kd of ~27 µM was measured (Komissarov et al., 1996; PMID: 8647821). The crystal structure of the Fab fragment in complex with dT5 was determined (Tanner et al., 2001; PMID: 11733999). Isothermal titration calorimetry experiments were used to measure the apparent affinity of the antibody for dT5. The apparent association constant was K_A = 6.0 × 10⁴ M^-1. Furthermore, the binding of dT5 to the antibody was exothermic, with an apparent ΔH_cal under solution conditions of -16.8 kcal/mol (Schuermann et al., 2004; PMID: 15340914). The crystal structure of the Fab version of this antibody was characterized in complex with a DNA ligand corresponding to nucleotides 5–14 of LIG1-17 (LIG5-14) (Ou et al., 2007; PMID: 18028946).

Product Class:	Purified
Clone ID:	DNA-1
Heavy Chain Modification:	Fc Silent™
Synonyms:	dT5; Oligo(dT); Oligo (dT); poly(dT); poly (dT)
Amount:	200 μg
Application Codes Clone:	IP; ELISA; Crystallography; ITC
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	Fab
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:	This antibody is specific for dT5, with high specificity to sequences greater than 5 nucleotides. It primarily recognizes the thymine DNA base, but it also binds to oligo(dT)15 nucleotides or greater and oligo(dT) of length three to five nucleotides. The antibody also recognizes the abbreviated hairpin sequence LIG5-14.
Application Notes:	The original format of this antibody (Fab) was generated and characterized. It was shown to bind DNA in an IP assay. and its binding affinity was determined to be (4.4±2.4) × 10^-8 M (Calcutt et al., 1993; PMID: 7506692). Fluorescence titration of the antibody with dT5 was performed, and a Kd of ~27 µM was measured (Komissarov et al., 1996; PMID: 8647821). The crystal structure of the Fab fragment in complex with dT5 was determined (Tanner et al., 2001; PMID: 11733999). Isothermal titration calorimetry experiments were used to measure the apparent affinity of the antibody for dT5. The apparent association constant was K_A = 6.0 × 10⁴ M^-1. Furthermore, the binding of dT5 to the antibody was exothermic, with an apparent ΔH_cal under solution conditions of -16.8 kcal/mol (Schuermann et al., 2004; PMID: 15340914). The crystal structure of the Fab version of this antibody was characterized in complex with a DNA ligand corresponding to nucleotides 5–14 of LIG1-17 (LIG5-14) (Ou et al., 2007; PMID: 18028946).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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