Vector Labs Ab00421-10.7-BS Anti-Type IV collagenase [3G11], Human F(ab)2, AbFab2™ His-Tagged, kappa (1 mg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: 3G11
Heavy Chain Modification: AbFab2™ His-Tagged
Synonyms: MMP2; MMP-2; MMP 2; TBE-1; Matrix Metalloprotease 2; 72 kDa type IV collagenase; 72 kDa gelatinase; Gelatinase A
Amount: 1 mg
Application Codes Clone: ELISA; Immunoscintigraphy; MTT assay; IHC; IF; in vivo
Shipping Temperature: Wet Ice
Origin Pub PMID: 20303650
Buffer Composition: PBS only.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody binds specifically to human and mouse type IV collagenase and does not show any cross-reactivity with any other collagenases. Human type IV collagenase, a metalloproteinase involved in degrading matrix proteins and hence contributing largely to metastasis in various types of cancer.
Application Notes: The specificity of the original format of this antibody (mouse IgG1, κ) was measured by ELISA analysis. The (131)I-labeled version of this antibody was demonstrated to be stable in vitro for almost 72 h. The blood clearance pattern of (125)I-labeled 3G11 was defined as a two-compartment model, with a 7.2 h T1/2α and a 345.2 h T1/2β. A clear scintigraphic image of human lung carcinoma PG was obtained using (131)I-labeled 3G11 at 72 h, which further improved at 120 h (Dai et al., 2003; PMID: 14693044). Lidamycin (LDM) was conjugated to the Fab version of this antibody, and the resulting immunoconjugate maintained most of its immunoreactivity to both type IV collagenase and mouse hepatoma 22 cells, as shown by ELISA, and exhibited more potent cytotoxicity to hepatoma 22 cells than free LDM by MTT assay. Furthermore, when administered intravenously, the Fab'-LDM conjugate was proven to be more effective against the growth of subcutaneously transplanted hepatoma 22 in mice than free LDM in two experiment settings, increasing mice survival time (Fengqiang et al., 2004; PMID: 15382678). The scFv version of this antibody was constructed and used in various configurations for IF and in vivo imaging and therapeutic applications (Zhong et al., 2010; PMID: 20303650) (Kong et al., 2015; PMID: 25824464).

Product Class:	Purified
Clone ID:	3G11
Heavy Chain Modification:	AbFab2™ His-Tagged
Synonyms:	MMP2; MMP-2; MMP 2; TBE-1; Matrix Metalloprotease 2; 72 kDa type IV collagenase; 72 kDa gelatinase; Gelatinase A
Amount:	1 mg
Application Codes Clone:	ELISA; Immunoscintigraphy; MTT assay; IHC; IF; in vivo
Shipping Temperature:	Wet Ice
Origin Pub PMID:	20303650
Buffer Composition:	PBS only.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody binds specifically to human and mouse type IV collagenase and does not show any cross-reactivity with any other collagenases. Human type IV collagenase, a metalloproteinase involved in degrading matrix proteins and hence contributing largely to metastasis in various types of cancer.
Application Notes:	The specificity of the original format of this antibody (mouse IgG1, κ) was measured by ELISA analysis. The (131)I-labeled version of this antibody was demonstrated to be stable in vitro for almost 72 h. The blood clearance pattern of (125)I-labeled 3G11 was defined as a two-compartment model, with a 7.2 h T1/2α and a 345.2 h T1/2β. A clear scintigraphic image of human lung carcinoma PG was obtained using (131)I-labeled 3G11 at 72 h, which further improved at 120 h (Dai et al., 2003; PMID: 14693044). Lidamycin (LDM) was conjugated to the Fab version of this antibody, and the resulting immunoconjugate maintained most of its immunoreactivity to both type IV collagenase and mouse hepatoma 22 cells, as shown by ELISA, and exhibited more potent cytotoxicity to hepatoma 22 cells than free LDM by MTT assay. Furthermore, when administered intravenously, the Fab'-LDM conjugate was proven to be more effective against the growth of subcutaneously transplanted hepatoma 22 in mice than free LDM in two experiment settings, increasing mice survival time (Fengqiang et al., 2004; PMID: 15382678). The scFv version of this antibody was constructed and used in various configurations for IF and in vivo imaging and therapeutic applications (Zhong et al., 2010; PMID: 20303650) (Kong et al., 2015; PMID: 25824464).

Specifications

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Brand	Vector Labs
UNSPSC	12161500

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