Mfg Part Number
Ab00430-10.3
Vector Labs Ab00430-10.3 Anti-Human Ephrin Type A receptor 2 [1C1], Human IgG1, Fc Silent™, kappa (200 μg)
Quick Overview
1C1 was isolated after screening of a non-immunized human Fab phage library. Selection was based on the ability to bind soluble human recombinant EphA2 (residues 183-510). The Fab was subsequently converted to full length human IgG1.
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| Product Class: | Purified |
|---|---|
| Clone ID: | 1C1 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | EphA2; Epithelial cell kinase; Tyrosine-protein kinase receptor ECK |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; FC; WB; IF; agonist; internalizing |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | 1C1 binds specifically to the amino-termianal LBD (ligand-binding domain) region of the EphA2 ectodomain (IgG1 KD ~0.8 nM; Fab KD ~140 nM) and not to other related human Eph receptors such as EphA1, and EphA4, or any EphB receptor family members. It is reported to cross react with mouse EphA2. |
| Application Notes: | This antibody binds to human Ephrin type-A receptor 2, a receptor tyrosine kinase that binds to membrane-bound ephrin-A ligands residing on adjacent cells, triggering contact-dependent signaling into neighboring cells. Eph2A is involved in pattern formation during development, angiogenesis and osteoblastogenesis. Eph2A has also been found to be expressed in a variety of cancers. This antibody acts as an agonist of Eph2A signaling. The agonist properties of 1C1 is due to ligand mimicry by VDR3. After cell binding, the antibody is able to induce rapid tyrosine phosphorylation, internalization, and degradation of the EphA2 receptor. |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |