Mfg Part Number
Ab00461-1.6
Vector Labs Ab00461-1.6 Anti-Y108F-LeuT [2B12], Mouse Fab fragment, His-Tagged, kappa (200 μg)
Quick Overview
Purified LeuT in detergent.
$595.40
Order will ship within 1-3 business days
| Product Class: | Purified |
|---|---|
| Clone ID: | 2B12 |
| Heavy Chain Modification: | His-Tagged |
| Synonyms: | Bacterial Leucine Transporter |
| Amount: | 200 μg |
| Application Codes Clone: | WB; FSEC |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2a |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques. |
| Application Notes: | The antibody binds specifically to LeuT is a bacterial symporter specific for small hydrophobic amino acids such as leucine and alanine, driven by the sodium electrochemical gradient across the membrane. The antibody is specific for the outward-open conformation (substrate-free/ sodium-bound states) of LeuT, which is stabilised by mutating Tyr108 to Phe in TM3 (Y108F_LeuT). The 2B12 antibody was selected to bind to this conformational state by fluorescence-detection size-exclusion chromatography (FSEC) and western Blot. The Fab fragment of the antibody was obtained by papain digestion. LeuT is a bacterial symporter specific for small hydrophobic amino acids such as leucine and alanine, driven by the sodium electrochemical gradient across the membrane. Due to the high homology of LeuT to human neurotransmitter transporters, its crystal structure can reveal the mechanism of action of many drugs, such as antidepressants. |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |