Mfg Part Number
Ab00462-1.6
Vector Labs Ab00462-1.6 Anti-TSY-LeuT [6A10], Mouse Fab fragment, His-Tagged, kappa (200 μg)
Quick Overview
Purified LeuT in detergent.
$595.40
Order will ship within 1-3 business days
| Product Class: | Purified |
|---|---|
| Clone ID: | 6A10 |
| Heavy Chain Modification: | His-Tagged |
| Synonyms: | Bacterial Leucine Transporter |
| Amount: | 200 μg |
| Application Codes Clone: | WB; FSEC |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Application Notes: | The antibody binds specifically to LeuT is a bacterial symporter specific for small hydrophobic amino acids such as leucine and alanine, driven by the sodium electrochemical gradient across the membrane. The antibody is specific for the inward-open conformation of LeuT, which required weakening of the Na2 binding site by mutating Thr 354 to Val, and Ser 355 to Ala in TM8, and the mutation of Tyr 268 to Ala in TM6 of the cytoplasmic gate. (TSY_LeuT). The Fab fragment of the antibody was obtained by papain digestion. Due to the high homology of LeuT to human neurotransmitter transporters, its crystal structure can reveal the mechanism of action of many drugs, such as antidepressants. |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |