Mfg Part Number
Ab00472-201.1

Vector Labs Ab00472-201.1 Anti-CD28 [1C6], Dog IgG2 (IgG-B), kappa (200 μg)

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Canine CD28.
$517.40
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Product Class:Purified
Clone ID:1C6
Synonyms:TP44; Costimulatory molecule B7 receptor CD28; T-cell costimulatory molecule CD28
Amount:200 μg
Application Codes Clone:FC; FA; in vivo; blocking; ELISA; activation
Shipping Temperature:Wet Ice
Buffer Composition:PBS with 0.02% Proclin 300.
Original Format:IgG1
Storage Temperature:Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:This chimeric dog antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:The antibody binds specifically to canine CD28, a self-surface receptor expressed by thymocytes and a subset of natural killer cells. CD28 acts as a receptor for CD80 and CD86, providing a costimulatory signal for the activation of T-cells and the production of interleukins, such as IL-6.
Application Notes:This antibody exhibits an antagonistic activity, blocking mixed lymphocyte reactions and cytotoxic T lymphocyte-mediated killing. The use of this antibody to neutralize the CD26 signal following T-cell receptor activation is of clinical importance for transplantation therapy, as it has been shown to prevent graft rejection and graft-versus-host disease in the canine model of allogeneic hematopoietic cell transplantation (HCT). Interestingly, this antibody also exhibits agnostic activity; it was coated onto magnetic beads and used for the activation of canine peripheral blood lymphocytes (Szopa et al., 2021; PMID: 33679741).
More Information
Brand Vector Labs
UNSPSC 12161500