Mfg Part Number
Ab00492-10.6
Vector Labs Ab00492-10.6 Anti-LeY [BR96], Human Fab fragment, His-Tagged, kappa (200 μg)
Quick Overview
H3396 cultured line established from a metastasis of a human breast adenocarcinoma.
$595.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | BR96 |
| Heavy Chain Modification: | His-Tagged |
| Synonyms: | Lewis Y |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; IHC |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 7664109 |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG3 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG3 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | The antibody binds Le(Y) with a Ka of 0.1 μM. |
| Application Notes: | The antibody binds specifically to LeY tetrasaccharide, a carbohydrate antigen expressed at the cell surface of selective carcinomas, including those of the colon, breast, ovary and lung. Studies have shown that the monoclonal antibody has toxic effects to antigen-positive tumour cells, performing ADCC at concentrations down to 0.1 μg/ml. The antibody B396 was also shown to perform complement-dependent cytotoxicity at concentrations of 0.1-5.0 μg/ml in certain carcinoma cell lines. The monoclonal antibody also has the ability to be internalised by antigen-positive tumour cells, and can thereby be used to deliver anti-cancer drugs to the specific tumour cells. |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |