Mfg Part Number
Ab00508-10.0
Vector Labs Ab00508-10.0 Anti-Botulinum neurotoxin [MCS6-27], Human IgG1, kappa (200 μg)
Quick Overview
Recombinant BoNT/B fragments expressed in E. coli as GST-fusion proteins.
$517.40
Order will ship within 1-3 business days
| Product Class: | Purified |
|---|---|
| Clone ID: | MCS6-27 |
| Synonyms: | BBoNT/B |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; WB |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 20548779 |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Application Notes: | The antibody binds specifically to BoNT/B, a two-chain neurotoxic protein produced by the bacterium Clostridium botulinum. Botulism, an infection of the bacterium, which is commonly spread through contaminated food, can be a fatal disease, and symptoms include a gradual flaccid paralysis. Paralysis is caused by the internalization by endocytosis of the toxin in specific axons, mediated by the attachment of the heavy chain of the protein to axon surface. Once inside the axon, BoNT/B acts by cleaving SNARE proteins, such as VAMP, which are involved in the docking and fusion of neurosecretory vesicles. This prevents the release of the neurotransmitter acetylcholine, resulting in paralysis of the muscles. The monoclonal antibody MCS6-27 binds to the Hc and H5 fragments of BoNT/B, and the binding epitope is located in the heavy chain, between amino acids E859 and E1291. The monoclonal antibody is able to capture BoNT/B at SDS concentrations between 0 and 0.4 mM, and can be used to screen food samples for botulinum toxin. |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |