Mfg Part Number
Ab00579-1.1
Vector Labs Ab00579-1.1 Anti-N-ethylmaleimide cysteine [OX-133], Mouse IgG1, lambda (200 μg)
Quick Overview
Purified MBP-gp120 (HIV-1) reduced using tris(2-carboxyethyl)phosphine (TCEP, 2.5 mM) for 20 minutes and alkylated using NEM (5 mM solubilized in PBS) for 30 minutes.
$517.40
Order will ship within 1-3 business days
| Product Class: | Purified |
|---|---|
| Clone ID: | OX-133 |
| Synonyms: | NEM cysteine; OX133 |
| Amount: | 200 μg |
| Application Codes Clone: | IP; ELISA; FC |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | This antibody recognises cysteine-boud N-ethylmaleimide in a sequence-independent manner. It has no affinity for unmodified peptides or peptides modified with other alkylating agents such as iodoacetamide. |
| Application Notes: | OX133 detects NEM-modified proteins and can be used as an affinity matrix to purify NEM-modified proteins from cell lysates. OX133 does not cross react with any other alkylating agent, making it a highly selective reagent for the purification of NEM-labeled proteins and potentially peptides for mass spectrometry-based analysis. |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |