JavaScript seems to be disabled in your browser. For the best experience on our site, be sure to turn on Javascript in your browser.

Skip to the beginning of the images gallery

Mfg Part Number

Ab00585-8.4

Vector Labs Ab00585-8.4 Anti-CD53 [OX-44], Rat IgG2b, Fc Silent™, kappa (200 μg)

VECTOR LABS

Be the first to review this product

Quick Overview

This antibody was prepared by immunizing BALB/c mice with T lymphoblasts from an MLR between purified rat T helper cells and irradiated semiallogeneic rat spleen.

$517.40

Order will ship within 1-3 business days

Description

Product Class: Purified
Clone ID: OX-44
Heavy Chain Modification: Fc Silent™
Synonyms: Leukocyte surface antigen CD53; Cell surface glycoprotein CD53; Leukocyte antigen MRC OX-44
Amount: 200 μg
Application Codes Clone: FC; IHC-Fr; IHC-P; IP
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rat antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody recognises CD53, which is expressed on peripheral T-cells, single (CD4 or CD8) positive thymocytes, B-cells, monocytes, macrophages, neutrophils, dendritic cells and microglial cells. This antibody recognises a conformational epitope that is dependent on disulphide bonding.
Application Notes: This antibody binds rat CD53, and consequently labels all myeloid cells and peripheral lymphoid cells, but only 12% of thymocytes (Paterson, 1987). Functional studies indicate that this subset of OX-44+ thymic cells includes all thymocytes that are on the pathway to immunocompetent T lymphocytes. By contrast, the OX-44- compartment represents cells that are negative for proliferation and thymopoiesic activity, and are fated to die in situ. In addition, this antibody has been used in conjunction with CD4 and CD8 antigens to define seven subsets of thymic cell. Most CD4-CD8- cells are OX-44+, whereas almost all CD4+ CD8+ cells were OX-44-

Product Class:	Purified
Clone ID:	OX-44
Heavy Chain Modification:	Fc Silent™
Synonyms:	Leukocyte surface antigen CD53; Cell surface glycoprotein CD53; Leukocyte antigen MRC OX-44
Amount:	200 μg
Application Codes Clone:	FC; IHC-Fr; IHC-P; IP
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rat antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody recognises CD53, which is expressed on peripheral T-cells, single (CD4 or CD8) positive thymocytes, B-cells, monocytes, macrophages, neutrophils, dendritic cells and microglial cells. This antibody recognises a conformational epitope that is dependent on disulphide bonding.
Application Notes:	This antibody binds rat CD53, and consequently labels all myeloid cells and peripheral lymphoid cells, but only 12% of thymocytes (Paterson, 1987). Functional studies indicate that this subset of OX-44+ thymic cells includes all thymocytes that are on the pathway to immunocompetent T lymphocytes. By contrast, the OX-44- compartment represents cells that are negative for proliferation and thymopoiesic activity, and are fated to die in situ. In addition, this antibody has been used in conjunction with CD4 and CD8 antigens to define seven subsets of thymic cell. Most CD4-CD8- cells are OX-44+, whereas almost all CD4+ CD8+ cells were OX-44-

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

Reviews