Mfg Part Number
Ab00586-8.1
Vector Labs Ab00586-8.1 Anti-CD43 [OX-58], Rat IgG2b, kappa (200 μg)
Quick Overview
This antibody was prepared by immunizing BALB/c mice with rat thymocyte leukosialin and thymocytes.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | OX-58 |
| Synonyms: | Leukosialin; Leukocyte sialoglycoprotein; Sialophorin; W3/13 antigen; OX 58; MRC OX 58 |
| Amount: | 200 μg |
| Application Codes Clone: | WB; FC; RIA |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2b |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rat antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody recognises rat leukosialin, a major cell-surface sialoglycoprotein of thymocytes and T lymphocytes. It is also expressed on neutrophils, bone marrow stem cells and B-lineage cells after activation. The specificity of the leukosialin epitope is largely determined by linear protein sequence, as indicated by the reaction of this antibody with the extracellular domain of leukosialian expressed in an unglycosylated form in E. coli. However, the sensitivity of this antibody to neuraminidase treatment of leukosialin indicates that glycosylation can modify epitope conformation or flexibility. |
| Application Notes: | This antibody binds to rat leukosialin, and can be used to label thymocytes and T lymphocytes in the lymph node cell population in flow cytometry analysis. This antibody has also been used, along with other anti-CD43 MABs, in a Western blot analysis to localize leukosialin epitopes. |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |