Mfg Part Number
Ab00587-8.4
Vector Labs Ab00587-8.4 Anti-CD4 [OX-68], Rat IgG2b, Fc Silent™, kappa (200 μg)
Quick Overview
This antibody was prepared by subcutaneously injecting BALB/c mice with a soluble form of rat CD4, consisting of domains 3 and 4. A boost of a mixture of free sCD4 and sCD4 bound to cells was administered intravenously.
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| Product Class: | Purified |
|---|---|
| Clone ID: | OX-68 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | Leu3/T4; T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3; W3/25 antigen |
| Amount: | 200 μg |
| Application Codes Clone: | FC; WB; IP; SPR; binding assay |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2a |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rat antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is reactive with rat CD4 domain 3 & 4, and is partly competitive with OX-67 & 69. |
| Application Notes: | This antibody binds to rat CD4. Upon challenge with HIV-1, this antibody was able to significantly inhibit viral entry into HeLa cells expressing a mutant of rat CD4 that binds gp120 (SImon, 1997). Similarly, pre-binding of gp120 resulted in a 50% reduction in binding of this antibody, indicating epitope masking. Analogous effects on viral entry and synctium formation are observed with MAbs against human CD4. |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |