Mfg Part Number
Ab00589-10.6
Vector Labs Ab00589-10.6 Anti-SIRP g [OX-119], Human Fab fragment, His-Tagged, kappa (200 μg)
Quick Overview
OX-119 was prepared by immunizing BALB/c mice with a chimeric protein made up of human SIRPg and a leader peptide of rat CD4 domain (SIRPg-CD4 ).
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| Product Class: | Purified |
|---|---|
| Clone ID: | OX-119 |
| Heavy Chain Modification: | His-Tagged |
| Synonyms: | CD172g; OX119; MRC OX-119; MRC OX119; SIRP-g; SIRPg; SIRP gamma; SIRP-gamma; CD172 antigen-like family member B; signal-regulatory protein beta; CD172-g |
| Amount: | 200 μg |
| Application Codes Clone: | IP; FC; ELISA; block |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | Hybridoma supernatants were initially screened by ELISA, using SIRPg-CD4, human SIRPa-Fc, human SIRPb-Fc or CD4 to eliminate hybridomas with cross-reactivity to rat CD4, human SIRPa, and SIRPb. There is some marginal binding of OX-119 to SIRPb transfected 293T cells. SIRPg binds CD47. On binding with CD47, mediates cell-cell adhesion. Engagement on T-cells by CD47 on antigen-presenting cells results in enhanced antigen-specific T-cell proliferation and costimulates T-cell activation. Expressed on CD4+ T-cells, CD8+ T-cells, CD56-bright natural killer (NK) cells, CD20+ cells, and all activated NK cells.. |
| Application Notes: | OX-119 is able to perform staining of peripheral blood myeloid cells (PBMCs) and block SIRPg-mediated apoptosis. OX-119 can also be used in IP, FC and ELISA. |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |