Mfg Part Number
Ab00592-3.0

Vector Labs Ab00592-3.0 Anti-CD43 [OX-56], Mouse IgG2b, kappa (200 μg)

Quick Overview
OX-56 was prepared by immunizing BALB/c mice with activated rat T lymphocytes.
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Product Class:Purified
Clone ID:OX-56
Synonyms:Leukosialin; OX56; MRC OX-56; MRC OX56; sialophorin
Amount:200 μg
Application Codes Clone:WB; IP
Shipping Temperature:Wet Ice
Buffer Composition:PBS with 0.02% Proclin 300.
Original Format:IgG2b
Storage Temperature:Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:OX-56 binds specifically to CD43 and binding is not affected by CD43 glycosylation. OX-56 reacts with most thymocytes and T lymphocytes in the lymph node cell population. This antibody also binds to pre-B cells and can detect CD43 at low levels on B cells. CD43 plays a role in the physicochemical properties of the T-cell surface and in lectin binding. CD43 is a major cell-surface sialoglycoprotein - also found on neutrophils, bone marrow stem cells, B-lineage cells after activation of thymocytes and T lymphocytes but is not expressed on resting B lymphocytes. The protein presents carbohydrate ligands to selectins and Is a counter-receptor for SN/Siglec-1. During T-cell activation it is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response.
Application Notes:OX-56 has been used in the purification of rat leukosialin by immunoaffinty chromatography (Willis et al. 1987) as well as in WB.
More Information
Brand Vector Labs
UNSPSC 12161500