Mfg Part Number
Ab00601-201.1
Vector Labs Ab00601-201.1 Anti-CD8A [YCATE55.9], Dog IgG2 (IgG-B), kappa (200 μg)
Quick Overview
This antibody was prepared by immunizing rats with canine leucocytes, thymocytes or concanavalin A-activated lymphoblasts.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | YCATE55.9 |
| Synonyms: | LEU2; T-cell surface glycoprotein CD8 alpha chain; CD8a |
| Amount: | 200 μg |
| Application Codes Clone: | Blocking; FC; IF; IHC-fr; fixed lymphocyte reaction (MLR); cell-mediated lympholysis assays. |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric dog antibody was made using the variable domain sequences of the original Rat IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for the alpha chain of canine CD8, and reacts with a rat cell line transfected with cDNA for canine CD8α (Gorman, 1994) |
| Application Notes: | This antibody recognizes canine CD8α, and has been used in in IF and IHC studies to establish that CD8 expression is confined to a minority population of T cells, as is the case for other species (Cobbold, 1994). In a canine transplant model, administration of this antibody, in combination with another anti-CD8 antibody (YCATE60.3.9) causes a 90 per cent depletion of CD8+ T cells from the peripheral blood, but does not result in prolonged allograft survival (Watson, 1993). However, the administration of this antibody in combination with an anti-CD4 antibody is associated with an immunosuppressive effect, and an extended median graft survival time. This antibody blocks MHC class I dependant T-cell responses in vitro and in vivo. |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |