Mfg Part Number
Ab00612-7.4
Vector Labs Ab00612-7.4 Anti-Cisplatin modified DNA [CP9/19], Rat IgG2a, Fc Silent™, kappa (200 μg)
Quick Overview
The original antibody was generated by immunizing F344 rats with cisplatin-modified native DNA.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | CP9/19 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | ICR4 |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; IHC; IP; Dot blot assay; IF |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2a |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | Antibody CP9/19 recognises only the intra-strand cross-links formed by cisplatin between adjacent purines. |
| Application Notes: | This antibody binds to cisplatin-modified DNA and enables the quantification of cisplatin-induced adducts on DNA (Tilby et al., 1991; PMID: 1703029). This antibody has also been used for the isolation of DNA fragments carrying adducts to enhance the sensitivity of subsequent PCR-based analyses and is central to ongoing studies of variation in the nature of cisplatin adducts formed in different cell lines (McGurk et al., 2001; PMID: 11494859). This antibody has been used for IHC on mouse samples (Rainey et al., 2016; PMID: 27122034). This antibody was used in an IF assay to detect platinum-DNA adducts (Nicholson et al., 2022; PMID: 35778553). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |