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Mfg Part Number

Ab00628-7.4

Vector Labs Ab00628-7.4 Anti-CD44 [Hermes-1], Rat IgG2a, Fc Silent™, kappa (200 μg)

VECTOR LABS

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Quick Overview

This antibody was produced by immunizing a Fischer rat with human tonsil lymphocytes (10^7cells intraperitoneally in Freund's complete adjuvant). A boost was given 2 weeks later (intravascularly in saline).

$517.40

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Description

Product Class: Purified
Clone ID: Hermes-1
Heavy Chain Modification: Fc Silent™
Synonyms: CDw44; Epican; Extracellular matrix receptor III; ECMR-III; GP90 lymphocyte homing/adhesion receptor; HUTCH-I; Heparan sulfate proteoglycan; Hermes antigen; Hyaluronate receptor; Phagocytic glycoprotein 1; PGP-1; Phagocytic glycoprotein I; CD44/H-CAM antibody; Hermes 1
Amount: 200 μg
Application Codes Clone: Blocking; ELISA; FC; ICC; IF; IHC; IP; Neutralization; WB
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG2a
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement: This antibody is specific for CD44 (gp90 epitope cluster I), which is selectively expressed on normal or transformed lymphoid cells that are able to recognize and bind to high endothelial venules (specialized vessels that mediate lymphocyte exit from the blood into lymphoid organs). Although this antibody was raised against human CD44, it cross-reacts with murine, rat, rabbit, primate, sheep, bovine and goat CD44.
Application Notes: This antibody binds CD44, so can be used to label CD44+ cells in flow cytometry analysis. As CD44 molecules are used to bind to high endothelial venules (HEV) at the site of lymphoctye entry to lymphoid organs, this antibody can be used to specifically stain HEV-binding cell lines (Jalkanen, 1986). In addition, this antibody blocks the adhesion of the haematopoietic progenitor cell line KG1 to hyaluronic acid (Bendall, 2003), but does not block lymphocyte binding to HEV (Picker, 1989). 7H9D6 partially blocks the binding of this antibody.

Product Class:	Purified
Clone ID:	Hermes-1
Heavy Chain Modification:	Fc Silent™
Synonyms:	CDw44; Epican; Extracellular matrix receptor III; ECMR-III; GP90 lymphocyte homing/adhesion receptor; HUTCH-I; Heparan sulfate proteoglycan; Hermes antigen; Hyaluronate receptor; Phagocytic glycoprotein 1; PGP-1; Phagocytic glycoprotein I; CD44/H-CAM antibody; Hermes 1
Amount:	200 μg
Application Codes Clone:	Blocking; ELISA; FC; ICC; IF; IHC; IP; Neutralization; WB
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG2a
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:	This antibody is specific for CD44 (gp90 epitope cluster I), which is selectively expressed on normal or transformed lymphoid cells that are able to recognize and bind to high endothelial venules (specialized vessels that mediate lymphocyte exit from the blood into lymphoid organs). Although this antibody was raised against human CD44, it cross-reacts with murine, rat, rabbit, primate, sheep, bovine and goat CD44.
Application Notes:	This antibody binds CD44, so can be used to label CD44+ cells in flow cytometry analysis. As CD44 molecules are used to bind to high endothelial venules (HEV) at the site of lymphoctye entry to lymphoid organs, this antibody can be used to specifically stain HEV-binding cell lines (Jalkanen, 1986). In addition, this antibody blocks the adhesion of the haematopoietic progenitor cell line KG1 to hyaluronic acid (Bendall, 2003), but does not block lymphocyte binding to HEV (Picker, 1989). 7H9D6 partially blocks the binding of this antibody.

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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