Mfg Part Number
Ab00641-2.0
Vector Labs Ab00641-2.0 Anti-CMV glycoprotein gB [HCMV37], Mouse IgG2a, kappa (200 μg)
Quick Overview
The original antibody was generated by immunization of rabbit with recombinant HCMV gB-VAC virus.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | HCMV37 |
| Synonyms: | Human cytomegalovirus glycoproteins gB |
| Amount: | 200 μg |
| Application Codes Clone: | ICC; NTRL; ELISA; IF; IHC-Fr; IP |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 7772562 |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2a |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | This antibody binds envelope glycoprotein B of the human cytomegalovirus, that plays a role in host cell entry, cell to-cell virus transmission, and fusion of infected cells. |
| Application Notes: | This antibody immunoprecipitates gB from HCMV infected cells and neutralizes HCMV infectivity in vitro. Immunofluorescence was used to study whether HCMV gB synthesized in infected cells is transported to the cell surface (PMID: 3024973). Humanized version of this antibody was made by grafting technique. Significant restoration of binding was obtained by substitution of human VH amino acids Thr28, Phe29, Ser30 with murine residues Ser28, Ile29, Thr30, in conjunction with the position 94 change (PMID: 7772562). This antibody was used in detection of gB in an ELISA (PMID: 22836657). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |