Mfg Part Number
Ab00699-1.26
Vector Labs Ab00699-1.26 Anti-H3K4me3 [304M3-B], Mouse scFv-Fc, kappa (100 μg)
Quick Overview
The biotinylated peptide from histone H3 ARTKQTARKSTG-GYCD. The last four amino acids GYCD were added for biotinylation purposes.
$692.90
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| Product Class: | Purified |
|---|---|
| Clone ID: | 304M3-B |
| Synonyms: | trimethyl-Histone H3 (Lys4); histone H3; H3K4; Trimethylated Histone H3; Trimethylated Lys; Trimethylated Lys4; Histone H3 trimethylated at Lysine 4; post translational modification; histone post-translational modification |
| Amount: | 100 μg |
| Application Codes Clone: | IP; CHIP; ChIP-seq; IP-MS; ELISA; WB; IF |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 26862167 |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | scFv |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This is a long-neck scFv-Fc fusion protein. The long-neck format allows the two scFvs to bind to the antigen in an "antigen clasping" conformation. For more information please consult Hattori et al. 2016. |
| Specificity Statement: | This antibody is highly specific for Histone H3 trimethylated at lysine 4. It has been tested on human histone H3 (K562 cells), but owing to the high conservation of histones, is expected to cross-react with many other species. |
| Application Notes: | This antibody has been used in IP-studies and works in immunofluorescence and Western Blot. In an long-neck scFv-Fc-fusion format been shown to exhibit a high degree of specificity owing to an antigen-clasping binding mechanism (see Hattori et al. 2016). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |