Mfg Part Number
Ab00731-23.0
Vector Labs Ab00731-23.0 Anti-CD64 [H22], Rabbit IgG, kappa (200 μg)
Quick Overview
The original antibody was generated by cloning a single-chain fragment (scFv) derived from the murine anti-human CD64 monoclonal antibody (mAb) clone "m22". Clone m22 was originally described by Guyre et al. (1989).
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| Product Class: | Purified |
|---|---|
| Clone ID: | H22 |
| Synonyms: | FcγRI; High affinity immunoglobulin gamma Fc receptor I; IgG Fc receptor I; Fc-gamma RI; FcRI; Fc-gamma RIA |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; FC; toxicity assays; competition studies |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This is a chimeric rabbit IgG antibody based on the original human IgG1 antibody, created for improved compatibility with existing reagents, assays, and techniques. |
| Specificity Statement: | This antibody specifically targets human CD64, recognizing an epitope distinct from the Fc binding domain. |
| Application Notes: | This antibody can be bound to a toxin so that it displays specific cytotoxicity and thus can be used to eliminate AML cells selectively. This antibody was bound to a truncated version of the Pseudomonas exotoxin A (ETA'). The binding activity of the VH/VL antibody format was unaffected by the fusion of the m22(scFv) coding regions to the ETA' coding sequences. FC analysis indicated that this immunotoxin drove 41% of primary leukemia cells from a patient with CD64-positive AML into early apoptosis (Tur et al., 2003; PMID: 14679004). Similarly, in an in vivo mouse model, IHC analysis revealed efficient elimination of human CD64+ tumor cells in mouse organs (Tur et al., 2011; PMID: 21077160). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |