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Mfg Part Number

Ab00743-2.3

Vector Labs Ab00743-2.3 Anti-CD40 [A54 scFv], Mouse IgG2a, Fc Silent™, lambda (200 μg)

VECTOR LABS

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Quick Overview

A54 human anti-CD40 scFv was selected from the n-CoDeR phage display library after 4 rounds of selections using biotinylated CD40-Fcγ fusion protein as bait and subsequent capture of bound phages on streptavidin-caoted magnetic beads.

$517.40

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Description

Product Class: Purified
Clone ID: A54 scFv
Heavy Chain Modification: Fc Silent™
Synonyms: TNFRSF5; A54; Tumor necrosis factor receptor superfamily member 5; B cell surface antigen CD40; B-cell cell surface antigen CD40; B-cell surface antigen CD40; B cell surface antigen CD40; antigen CD40; CD40 antigen; Bp50; Bp-50; CD40L receptor CDw40
Amount: 200 μg
Application Codes Clone: ELISA; block; activate
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: scFv
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This full-length, chimeric mouse antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: A54 binds specifically to the D1 domain of CD40 (and can only bind to the intact, i.e., non-truncated version of CD40, and when an AD2 peptide tag has not been added) and can recognise CD40 on a B cell line (BJAB). The scFv version of this antibody binds CD40 (immobilized by amine coupling) with a KD of 40nM. CD40 is a glycoprotein of the TNFR superfamily and is expressed on all mature B cells, dendritic cells, activated monocytes, some endothelial cells, and some epithelium including the thymus. CD40 binds to the membrane protein CD154 of the TNF superfamily, and this interaction is required for the seconfary immune responses and germinal centre formation.
Application Notes: A54 is able to block the CD40-CD154 interaction by around 60-80% and can also induce CD40 sinalling in human B cells when at 'unusually high concentrations' (The CD40 receptor - target, tool and technology, Ellmark, 2002). This scFv can also be used for ELISA studies (PMID: 12153507)

Product Class:	Purified
Clone ID:	A54 scFv
Heavy Chain Modification:	Fc Silent™
Synonyms:	TNFRSF5; A54; Tumor necrosis factor receptor superfamily member 5; B cell surface antigen CD40; B-cell cell surface antigen CD40; B-cell surface antigen CD40; B cell surface antigen CD40; antigen CD40; CD40 antigen; Bp50; Bp-50; CD40L receptor CDw40
Amount:	200 μg
Application Codes Clone:	ELISA; block; activate
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	scFv
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This full-length, chimeric mouse antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	A54 binds specifically to the D1 domain of CD40 (and can only bind to the intact, i.e., non-truncated version of CD40, and when an AD2 peptide tag has not been added) and can recognise CD40 on a B cell line (BJAB). The scFv version of this antibody binds CD40 (immobilized by amine coupling) with a KD of 40nM. CD40 is a glycoprotein of the TNFR superfamily and is expressed on all mature B cells, dendritic cells, activated monocytes, some endothelial cells, and some epithelium including the thymus. CD40 binds to the membrane protein CD154 of the TNF superfamily, and this interaction is required for the seconfary immune responses and germinal centre formation.
Application Notes:	A54 is able to block the CD40-CD154 interaction by around 60-80% and can also induce CD40 sinalling in human B cells when at 'unusually high concentrations' (The CD40 receptor - target, tool and technology, Ellmark, 2002). This scFv can also be used for ELISA studies (PMID: 12153507)

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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