Mfg Part Number
Ab00744-23.0
Vector Labs Ab00744-23.0 Anti-NCAM-L1 [H2 scFv], Rabbit IgG, lambda (200 μg)
Quick Overview
single-chain variable fragment antibodies against mouse L1 from a human synthetic phage display library.
$517.40
Order will ship within 1-3 business days
| Product Class: | Purified |
|---|---|
| Clone ID: | H2 scFv |
| Synonyms: | CD171; L1; Neural cell adhesion molecule L1; N-CAM-L1; L1 |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; activate; WB; IF |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | scFv |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This full-length, chimeric rabbit antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This scFv binds specifically to the fibronectin type III repeats 1–2 of mouse L1 with a KD of around 2μM (determined by ELISA). It does not cross-react with human L1 in ELISAs, Western blots or immunofluorescence staining of cultured neurons. L1 plays important roles in cell adhesion, neuronal migration, neurite outgrowth, fasciculation and pathfinding, neuronal survival, and synaptic plasticity. L1 is not recongised under reducing conditions. L1 is a membrane glycoprotein of the immunoglobulin superfamily. Its interacts with adhesion molecules and proteins of the ECM. L1 also is inolved in the migration of cerebellar granule cells, enhancing neuronal survival and memory. |
| Application Notes: | This scFv can stimulate neurite outgrowth from cerebellar dorsal, rootganglion and motor neurons and also be used to enhance survival of cerevellar neurons by facilitating the clustering of L1. H2 can also be used for ELISA, WB and IF studies. |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |