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Mfg Part Number

Ab00746-10.6

Vector Labs Ab00746-10.6 Anti-Thrombopoietin [TN1 ], Human Fab fragment, His-Tagged, kappa (100 μg)

VECTOR LABS

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Quick Overview

TN1 was prepared by immunizing BALB/c mice by intraperitoneal injecction of recombinant human TPO. The Fab fragment was produced via incubation with papain which cleaves at the hinge region of the IgG to remove the Fc region. The product is then subsequently incubated under reducing conditions and the free Cys residue blocked with iodoacetamide.

$692.90

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Description

Product Class: Purified
Clone ID: TN1
Heavy Chain Modification: His-Tagged
Synonyms: C-mpl ligand; Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; TPO
Amount: 100 μg
Application Codes Clone: neutralize; cyrstallization; ELISA
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: TN1 binds specifically to TPO at an epitope which consists of residues from the C-terminal end of helix C (R98) and the C–D crossover loop (residues 102–115) and residues from the C-terminal half of helix B (residues 57–61) (PMID: 14769915). TPO is a heavily glycosylated cytokine which acts as the ligand for the hematopoietic receptor c-Mpl. The primary role of TPO is as a regulator of megakaryocytopoiesis and platelet production. Stimulated c-Mpl transduces signals into target cells via the JAK/STAT, Ras and MAPK pathways.
Application Notes: TN1 can neutralize the biological activity of TPO and can be used to detect TPO in ELISA studies. The Fab fragement of TN1 can also be used to aid the crystallisation of TPO by increasing the area upon which stable crystal contacts can be made (PMID: 11976502).

Product Class:	Purified
Clone ID:	TN1
Heavy Chain Modification:	His-Tagged
Synonyms:	C-mpl ligand; Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; TPO
Amount:	100 μg
Application Codes Clone:	neutralize; cyrstallization; ELISA
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	TN1 binds specifically to TPO at an epitope which consists of residues from the C-terminal end of helix C (R98) and the C–D crossover loop (residues 102–115) and residues from the C-terminal half of helix B (residues 57–61) (PMID: 14769915). TPO is a heavily glycosylated cytokine which acts as the ligand for the hematopoietic receptor c-Mpl. The primary role of TPO is as a regulator of megakaryocytopoiesis and platelet production. Stimulated c-Mpl transduces signals into target cells via the JAK/STAT, Ras and MAPK pathways.
Application Notes:	TN1 can neutralize the biological activity of TPO and can be used to detect TPO in ELISA studies. The Fab fragement of TN1 can also be used to aid the crystallisation of TPO by increasing the area upon which stable crystal contacts can be made (PMID: 11976502).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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