Mfg Part Number
Ab00750-23.0
Vector Labs Ab00750-23.0 Anti-osteopontin [hu1A12], Rabbit IgG, kappa (200 μg)
Quick Overview
1A12 antibody was prepared by immunizing BALB/c mice with purified recombinant human OPN. The antibody was humanized by complementarity-determining region grafting the mouse 1A12 CDRs into a human FR template, with some FR residues being substituted for those found to be key in the mouse version to maintain affinity for human OPN. The light and heavy chain expression vectors were co-transfected into COS-7 cells.
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| Product Class: | Purified |
|---|---|
| Clone ID: | hu1A12 |
| Synonyms: | OPN; Bone sialoprotein 1; Nephropontin; Secreted phosphoprotein 1; SPP-1; SPP1; Urinary stone protein; uropontin |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; IHC; SPR; inhibit |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 19690854 |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | hu1A12 binds to the epitope (212)NAPSD(216) of the human OPN protein sequence. High levels of OPN expression is associated with highly metastatic cancer cells and has been a target in cancer therapy. OPN has been shown to contribute to the malignancy of breast cancer as it is involved in pathways regulating cell adhesion and invasion, enhancing cell motility produces pro-survival signals by regulating the activities of Bcl-2 proteins. OPN possesses domains which facilitate interaction with multiple integrins and CD44 proteins. OPN is expressed in multiple cell types including osteoclasts, osteoblasts, epithelial cells of the breast, kidney, skin, nerve cells, vascular smooth muscle cells, endothelial cells and activated macrophages and lymphocytes. |
| Application Notes: | hu1A12 suppreses MDA-MB-435S (highly metastatic human breast cancer cell line) cell migration (evaluated by scratch-wound healing) and also inhibits cell adhesion of MDA-MB-435S cells to human OPN. hu1A12 also inhibits the anti-apoptotic and pro-survival functions of OPN possibly through upregulating Bax, downregulating Bcl-2, and blocking the activation of NF-κB. hu1A12 also has been shown to suppress primary breast tumour growth in humans through in vivo studies. This antibody has also been used in ELISA, IHC and SPR studies. |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |