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Mfg Part Number

Ab00751-1.1

Vector Labs Ab00751-1.1 Anti-MRP1 [A5 scFv], Mouse IgG1, kappa (200 μg)

VECTOR LABS

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Quick Overview

A5 scFv was isolated from a phage display. The source of the antibody genes came from the spleens of mice which had been immunized with f88 filamentous bacteriophages displaying a peptide derived from the extracellular N-terminus (aa 1-18) of human MRP1. The scFv A5 was expressed in BL21 E. coli cells and purified from the periplasmic fraction by metal-ion affinity chromatography.

$517.40

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Description

Product Class: Purified
Clone ID: A5 scFv
Synonyms: LTC4 transporter; Multidrug resistance-associated protein 1; MRP-1; MRPI; MRP-I; ATP-binding cassette sub-family C member 1; Leukotriene C(4) transporter; LTC4 transporter
Amount: 200 μg
Application Codes Clone: FC; ELISA
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: scFv
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This full-length, reformatted mouse antibody was made using the variable domain sequences of the original Mouse scFv format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: A5 scFv binds specifically to the extracellular N-terminus of MRP1, with no cross-reactivity with other MRPs or BCRP. FC analysis showed that A5 reacts strongly with viable ovarian carcinoma 2008/MRP1 and small cell lung carcinoma GLC4/ADR cells that overexpress MRP1. MRP1 is a member of the ABC superfamily of transporter proteins and confers resistance to various toxic compounds and are involved in multi-drug resisitance in chemotherapy. This protein mediates the export of organic anions and drugs from the cytoplasm.
Application Notes: standard and saturation ELISA. The avidity of the scFv wsa increased for FC studies through tetramerization of A5 by adding a BirA tag to the C terminus for site-specific biotinylation and tetramerized using PE-labelled streptavidin.

Product Class:	Purified
Clone ID:	A5 scFv
Synonyms:	LTC4 transporter; Multidrug resistance-associated protein 1; MRP-1; MRPI; MRP-I; ATP-binding cassette sub-family C member 1; Leukotriene C(4) transporter; LTC4 transporter
Amount:	200 μg
Application Codes Clone:	FC; ELISA
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	scFv
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This full-length, reformatted mouse antibody was made using the variable domain sequences of the original Mouse scFv format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	A5 scFv binds specifically to the extracellular N-terminus of MRP1, with no cross-reactivity with other MRPs or BCRP. FC analysis showed that A5 reacts strongly with viable ovarian carcinoma 2008/MRP1 and small cell lung carcinoma GLC4/ADR cells that overexpress MRP1. MRP1 is a member of the ABC superfamily of transporter proteins and confers resistance to various toxic compounds and are involved in multi-drug resisitance in chemotherapy. This protein mediates the export of organic anions and drugs from the cytoplasm.
Application Notes:	standard and saturation ELISA. The avidity of the scFv wsa increased for FC studies through tetramerization of A5 by adding a BirA tag to the C terminus for site-specific biotinylation and tetramerized using PE-labelled streptavidin.

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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