Mfg Part Number
Ab00754-1.1
Vector Labs Ab00754-1.1 Anti-Fc gamma receptor III [3G8], Mouse IgG1, kappa (200 μg)
Quick Overview
3G8 was prepared by immunizing mice with human PMN (polymorphonuclear leukocytes) cells.
$517.40
Order will ship within 1-3 business days
| Product Class: | Purified |
|---|---|
| Clone ID: | 3G8 |
| Synonyms: | CD16; low affinity IgG receptor III; FcγRIII; low affinity FcγRIII |
| Amount: | 200 μg |
| Application Codes Clone: | FC; SPR; depletion; IP; IHC; block |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | 3G8 binds to CD16 at an epitope which is not the IgG-binding site. This antibody cannot distinguish between CD16a transmembrane isoform found on the surface of NK cells and macrophages (which can trigger the cytolytic response), and the GPI-linked CD16b isoform present on neutrophils or soluble CD16 found in the plasma. CD16 is the low affinity IgG receptor III and binds to aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis and ADCC (antibody-dependent cell-mediated cytotoxicity). |
| Application Notes: | 3G8 antibody was used to construct a recombinant bispecific scFv (bsscFv) along with the mAb clone F3.3 anti-HLA class II antibody (connected by a (Gly4Ser)4 linker)which was expressed in SF21 insect cells. This bsscFv allows NK cells to target malignant B lymphoid cells and mediates effector cell lysis (ASCC) of the malignant cells (also lysis of primary leukaemia samples). 3G8 is also able to block neutrophil phagocytosis and stimulates NK cell proliferation, and can be used in FC, SPR, IP and IHC studies. |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |