Mfg Part Number
Ab00759-1.6
Vector Labs Ab00759-1.6 Anti-Acetylaminofluorene [K16-16], Mouse Fab fragment, His-Tagged, kappa (100 μg)
Quick Overview
K16-16 mAb was generated by immnizing BALB/c mice with acetyl aminofluorene-substituted guanosine (Le Guernet al, 1984). RNA derived from this hybridoma clone was then used to construct a phage display library and was selected for by its affinity for AAF-labelled sonicated salmon sperm DNA in an ELISA screen.
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| Product Class: | Purified |
|---|---|
| Clone ID: | K16-16 |
| Heavy Chain Modification: | His-Tagged |
| Synonyms: | 2-AAF; AAF |
| Amount: | 100 μg |
| Application Codes Clone: | Southern blot; dot blot hydridization; colony hybridization; plaque hybridization; PCR |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This reformatted mouse antibody was made using the variable domain sequences of the original Mouse scFv (IgG1) format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | K16-16 binds to AAF-labelled DNA. AAF is used to label DNA and RNA by binding covalently at the C8 position of guanosines; AAF can then be detected using anti-AAF antibodies. |
| Application Notes: | K16-16 was used to construct a recombinant conjugate by fusing PhoAv bacterial phosphatase. The conjugate was used for one-step immunodetection of DNAs and RNAs in dot-blot, colony and plaque hybridization and in Southern/northern blot hybridization. It has also been used to detect PCR products. |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |