Vector Labs Ab00761-10.6 Anti-LMP1 [H3], Human Fab fragment, His-Tagged, kappa (100 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: H3
Heavy Chain Modification: His-Tagged
Synonyms: latent membrane protein 1; LMP1; LMP-1; p63; protein p63; protein p25
Amount: 100 μg
Application Codes Clone: ELISA; IP; IF; inhibit
Shipping Temperature: Wet Ice
Origin Pub PMID: 17460414
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: scFv
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This reformatted human antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: H3 scFv is specific to the LMP1 C terminal region where it binds to CTAR-1 (GST-LMP1[187-242]). LMP1 is expessed constitutively in many EBV-associated tumours and this protein can cause B lymphocyte and rodent fibroblast transformation, cell apoptosis inhibition by up-regulation of anti-apoptotic proteins, cell surface marker and DNA methyltransferase activty up-regulation, and cell adhesion molecule and cyclin-dependent kinase downregulation. The C-terminal domain of LMP1 is made up of 3 C-terminal activating regions (CTARs) which interact with TNF receptor-associated proteins and TNF receptor-associated death domain protein, which medite activation of NF-kB and AP-1. A LMP1 TAR is also involved in the JAK/STAT pathway.
Application Notes: H3 scFv can reduce LMP1-mediated NF-kappaB activation in HEK293 cells by ~50% as well as inhibit LMP1 functions in epithelial cells - enables the potential use of H3 for attenuating the LMP1 function in LMP1-positive tumors. H3 is also capable of reducing the trans-membrane migration ability of MDCK-LMP1 cells by blocking the CTAR-mediated signaling pathway. H3 can also be used to pull down LMP1 in IPs, and can be used for ELISAs and to visualise LMP1 distibution in IF studies.

Product Class:	Purified
Clone ID:	H3
Heavy Chain Modification:	His-Tagged
Synonyms:	latent membrane protein 1; LMP1; LMP-1; p63; protein p63; protein p25
Amount:	100 μg
Application Codes Clone:	ELISA; IP; IF; inhibit
Shipping Temperature:	Wet Ice
Origin Pub PMID:	17460414
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	scFv
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This reformatted human antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	H3 scFv is specific to the LMP1 C terminal region where it binds to CTAR-1 (GST-LMP1[187-242]). LMP1 is expessed constitutively in many EBV-associated tumours and this protein can cause B lymphocyte and rodent fibroblast transformation, cell apoptosis inhibition by up-regulation of anti-apoptotic proteins, cell surface marker and DNA methyltransferase activty up-regulation, and cell adhesion molecule and cyclin-dependent kinase downregulation. The C-terminal domain of LMP1 is made up of 3 C-terminal activating regions (CTARs) which interact with TNF receptor-associated proteins and TNF receptor-associated death domain protein, which medite activation of NF-kB and AP-1. A LMP1 TAR is also involved in the JAK/STAT pathway.
Application Notes:	H3 scFv can reduce LMP1-mediated NF-kappaB activation in HEK293 cells by ~50% as well as inhibit LMP1 functions in epithelial cells - enables the potential use of H3 for attenuating the LMP1 function in LMP1-positive tumors. H3 is also capable of reducing the trans-membrane migration ability of MDCK-LMP1 cells by blocking the CTAR-mediated signaling pathway. H3 can also be used to pull down LMP1 in IPs, and can be used for ELISAs and to visualise LMP1 distibution in IF studies.

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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