Mfg Part Number
Ab00764-2.0
Vector Labs Ab00764-2.0 Anti-Urease alpha subunit [HpU-2], Mouse IgG2a, kappa (200 μg)
Quick Overview
HpU-2 was prepared by subcutaneous injection of purified H. pylori urease into BALB/c mice.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | HpU-2 |
| Synonyms: | urease-alpha; alpha-urease; urease |
| Amount: | 200 μg |
| Application Codes Clone: | Inhibit; SPR; ELISA |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | HpU-2 binds specifically to H. pylori at the epitope with sequence SVELIDIGGNRRIFGFNALVDR - the sequence is part of a loop near to the C-terminus of the alpha subunit of urease (the beta subunit possesses the active site). HpU-18 competes with HpU-2 for binding to this epitope. H. pylori is a gram negative spiral bacterium and causes numerous gastroduodenal diseases. It expresses large amounts of urease when present in the human stomach; urease is key for the survival and pathogenesis of the bacteria as it neutralizes the acidity in the stomach by hydrolysis of urea into ammonia. |
| Application Notes: | HpU-2 results in ~82% inhibition (allosteric inhibition) of urease activity and the inhibitory activity is increased to 87% when HpU-2 is used in combination with HpU-19. HpU-2 has been shown be unsuccessful for use in IHC of formalin-fixed sections of H. pylori-infected human cells. HpU-2 can also be used in SPR and ELISA studies. |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |