Mfg Part Number
Ab00765-10.6-BS
Vector Labs Ab00765-10.6-BS Anti-Butyrylcholinesterase [11D8], Human Fab fragment, His-Tagged, kappa (1 mg)
Quick Overview
11D8 wsa prepared by immunizing BChE -/- mice with sugar-diminished full-length recombinant human BChE (Hrabovska et al. 2010).
$5,162.30
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| Product Class: | Purified |
|---|---|
| Clone ID: | 11D8 |
| Heavy Chain Modification: | His-Tagged |
| Synonyms: | BChE |
| Amount: | 1 mg |
| Application Codes Clone: | ELISA; IP; IHC; WB |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 26343001 |
| Buffer Composition: | PBS only. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | 11D8 binds to both native and denatured human butyrylcholinesterase (HuBChE) with KD ~0.26 nM (determined by ELISA - 0.9 nM by SPR). Binding of 11D8 excludes binding of mAb B2 18-5, suggesting tht that bind to the same/similar epitope on huBChE. The antibody also binds to human albumin, which can compete with huBChE for binding. huBChE in plasma is a serine esterase that catalyzes the hydrolysis of esters including aspirin, cocaine, heroin, and octanoyl ghrelin. |
| Application Notes: | The percent active HuBChE bound to 11D8 in ELISAS is highest when the volume of plasma was lowest. 11D8 also binds to human albumin and so 11D8 is less suitable for immunopurification of huBChE from plasma compared to e.g. B2 18-5. |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |