Mfg Part Number
Ab00768-1.1
Vector Labs Ab00768-1.1 Anti-HBsAg [F124], Mouse IgG1, kappa (200 μg)
Quick Overview
BALB/c mice were immunized by injection of HBV particles serotype ay, purified from HBsAg-positive patient blood sera. Splenocytes from immunized mice were then fused with the Sp2/0-Ag myeloma cell line.
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| Product Class: | Purified |
|---|---|
| Clone ID: | F124 |
| Synonyms: | HBV M protein; Hepatitis B virus surface antigen; Hepatitis B surface antigen; HBV surface antigen; HBV-sAg; Hepatitis B virus M protein; Major surface protein |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; NTRL; RIA; WB |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 9891981 |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | F124 was shown to react with an epitope within the pre-S2 region of the HBV surface antigen M protein, shown by Western blot and RIA using both full viral particles and HBsAg particles (Budkowska et al, 1986). F124 is also reactive against a synthetic peptide corresponding to the N-terminal residues 120-150 (FPAGGSSSGTVNPVLTTASPL) of the pre-S2 region (Ni et al, 2010). Hepatitis B is one of the most common infectious diseases on a global scale, with infection associated with both acute and chronic liver inflammation and implicated in up to half of all occurring hepatocellular carcinomas. |
| Application Notes: | F124 was used as a solid-phase coating for RIAs detecting HBV particles, resulting in particle immobilisation by binding the pre-S2 epitope of the surface antigen (Budkowska et al, 1986). An scFv-formatted version of F124, comprising the same VH and VL sequences, showed dose-dependent binding to, and competitive blocking of IgG and Fab against, r-HBsAg in an ELISA (Passafiume et al, 1998). Size-exclusion HPLC indicates that, while the scFv monomer shows no tendency to aggregate at low concentrations, equilibrium is achieved at a monomer/dimer ratio of 3:1 at high protein concentrations. |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |