Mfg Part Number
Ab00772-3.3
Vector Labs Ab00772-3.3 Anti-Glycoprotein H [A13-d6.3], Mouse IgG2b, Fc Silent™, kappa (200 μg)
Quick Overview
d6.3 was generated by immunizing mice with PrV.
$517.40
Order will ship within 1-3 business days
| Product Class: | Purified |
|---|---|
| Clone ID: | A13-d6.3 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | d6.3; gH; Envelope glycoprotein H |
| Amount: | 200 μg |
| Application Codes Clone: | neutralize; crystalize; immunostaining |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2a |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | d6.3 Fab fragment binds to the core fragment of the PrV glycoprotein H (gH) ectodomain (aa 107–639) which is the membrane-proximal end of the protein in the ΔgL-Pass PrV variant of PrV. The antibody contacts residues 332-373 (peripheral helices α6 and α7 of domain III of gH contribute most of the residues that form the epitope recognized by d6.3). PrV is a porcine herpesvirus belonging to the alpha-Herpesvirinae subfamily and is known to cause Aukeszky's disease in pigs. gH is a type I membrane portein and can interact non-covalently with gL via the large gH ectodomain. |
| Application Notes: | d6.3 neutralizes the ΔgL-Pass PrV variant of PrV and can stain ΔgL-Pass PrV variant but not the wild-type PrV by immunostaining. The epitope of d6.3 is not blocked by the formation of the gH/gL heterodimer. The d6.3Fab fragment (obtained through papain digestion) can be complexed with the core fragment of the PrV glycoprotein H (gH) ectodomain (aa 107–639) for structural studies by crystallography. |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |