Mfg Part Number
Ab00774-2.0
Vector Labs Ab00774-2.0 Anti-Poxvirus L1 Protein [Fab 7d11], Mouse IgG2a, kappa (200 μg)
Quick Overview
This antibody was raised against Poxvirus L1 Protein.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | Fab 7d11 |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; PRNT; crystallization |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for a conformational epitope with Asp35 as the key residue on the poxvirus L1 protein. L1 is a myristoylated, transmembrane protein found on the surface of the mature virion that plays a key role in the formation of infectious virus. L1 is conserved in all orthopoxviruses, and its sequence is almost identical among vaccinia virus (VACV), Variola virus and monkeypox virus. |
| Application Notes: | The ability of this antibody to bind vaccinia virus has been confirmed using ELISA (Su, 2007). Both full -length and fab fragemtns of mAb-7D11, are able to neutralize VACV infection in plaque reduction–neutralization tests. However, 7D11-Fab required over 100 times greater concentrations of protein to give a similar level of neutralization to 7D11-F(ab′)2, indicating that bivalency plays a role in the capacity of mAb-7D11 to efficiently neutralize VACV. Finally, as the fab forms a stable complex with L1 protein, it has been used in X-ray crystallography studies to understand the basis of the conformational specificity of mAb-7D11 recognition (Su, 2007). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |