JavaScript seems to be disabled in your browser. For the best experience on our site, be sure to turn on Javascript in your browser.

Skip to the beginning of the images gallery

Mfg Part Number

Ab00777-1.6

Vector Labs Ab00777-1.6 Anti-alpha-2 integrin inserted-domain [2E10], Mouse Fab fragment, His-Tagged, kappa (100 μg)

VECTOR LABS

Be the first to review this product

Quick Overview

2E10 was generated by immunizing BALB/c mice with a DNA vaccine containing the human alpha2 I-domain cDNAsequence within pVAC2. The alpha2 I-domain is expressed as a fusion protein with fragment C of tetanus toxoid (FrC-TT). The scFv was then created using a V-gene phage display library derived from RNA from mice splenocytes and isolated following selection on GST-I after 3 rounds of panning.

$692.90

Order will ship within 1-3 business days

Description

Product Class: Purified
Clone ID: 2E10
Heavy Chain Modification: His-Tagged
Synonyms: CD49b; alpha2-beta1 integrin; glycoprotein IaIIa; GP IaIIa; VLA2; VLA-2; collagen receptor; alpha-2 integrin I-domain; integrin alpha-2; integrin alpha 2
Amount: 100 μg
Application Codes Clone: ELISA; FC
Shipping Temperature: Wet Ice
Origin Pub PMID: 16411412
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG (scFv)
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG (scFv) format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: scFv 2E10 can bind to the I (inserted) domain of recombinant alpha2 (GST- and His-), but cannot bind to the native epitope on platelets. This scFv also cross-reacts with and binds to the I-domain of alpha1 integrin. The alpha2 I-domain consists of ~200 amino acids and contains the ligand-binding site. The α2β1 integrin is expressed on platelets, fibroblasts, activated T lympho-cytes, endothelial cells and epithelial cells where its function is to act as a collagen and laminin receptor.
Application Notes: 2E10 scFv can be used to detect alpha2 and alpha1 intergin I-domain in ELISA and FC studies.

Product Class:	Purified
Clone ID:	2E10
Heavy Chain Modification:	His-Tagged
Synonyms:	CD49b; alpha2-beta1 integrin; glycoprotein IaIIa; GP IaIIa; VLA2; VLA-2; collagen receptor; alpha-2 integrin I-domain; integrin alpha-2; integrin alpha 2
Amount:	100 μg
Application Codes Clone:	ELISA; FC
Shipping Temperature:	Wet Ice
Origin Pub PMID:	16411412
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG (scFv)
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG (scFv) format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	scFv 2E10 can bind to the I (inserted) domain of recombinant alpha2 (GST- and His-), but cannot bind to the native epitope on platelets. This scFv also cross-reacts with and binds to the I-domain of alpha1 integrin. The alpha2 I-domain consists of ~200 amino acids and contains the ligand-binding site. The α2β1 integrin is expressed on platelets, fibroblasts, activated T lympho-cytes, endothelial cells and epithelial cells where its function is to act as a collagen and laminin receptor.
Application Notes:	2E10 scFv can be used to detect alpha2 and alpha1 intergin I-domain in ELISA and FC studies.

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

Reviews